P-201: Prevalence of 4977bp Deletion in Mitochondrial DNA in IVF Failure Women

Background: Successful IVF process is limited by factors such as oocyte quality. Oocyte quality can be defined as its abilities to be fertilized, mature and give rise to normal offspring and it is dependent on nuclear maturation and cytoplasm maturation. Damage to mitochondrial DNA (mtDNA) has been described in oocytes in IVF failure women that decrease cytoplasmic quality because Mitochondria are essential for respiration and oxidative phosphorylation of the cell. The presence of mtDNA 4977bp deletion in many different tissues can serve as a marker of this damage. However, no attempt has been made to detect the presence of mtDNA 4977bp in blood cells of these women. This is the first report concerning the 4977bp deletion in IVF failure women. Materials and Methods: Polymerase chain reaction techniques (PCR) were used to detect mtDNA 4977bp deletion in blood samples of 100 IVF failure women and compared with 100 women who had success IVF procedure. Results: The prevalence of 4977bp deletion in mtDNA was 88% in IVF failure women and 45% in control samples. Conclusion: The higher prevalence of mtDNA 4977bp deletion in patients in this study indicates that damage to mtDNA in blood cells can serve as a marker that demonstrate reduced quality of oocyte.